The fire began on Monday near Kibbutz Kalia, north of Israel's Dead Sea.
Thirteen injured people with varying degrees of injury, three of which were in moderate condition, were evacuated to medical centers in Haifa.
Four major fires broke out across Israel on Monday, with dozens of firefighting teams and multiple planes fighting against the flames.
The children from the Israeli Bedouin town of Tel Sheva were hurried to the hospital in serious condition but died there shortly after.
No foul play was suspected.
Firefighters were continuing to tackle the blaze and 80 people have been evacuated, according to the rescue services.
Ram Ben Barak's Moshav Nahalal barn burned down overnight, and electrical malfunctions have been ruled out as the cause.
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